In another study ( Van Loo et al. Muc1 null animals housed in a conventional facility were examined for visually apparent inflammation of the eye and surrounding tissue. INTRODUCTION Allergic conjunctivitis induced by pollen is one of the most widespread ocular diseases globally. Purpose: Twenty-four hours later, the mice were challenged with RW in 1995 Aug;36(9):1818-27. Prevention of allergic conjunctivitis in mice by a rice-based edible vaccine containing modified Japanese cedar pollen allergens Ken Fukuda,1 Waka Ishida,1 Yosuke Harada,1 Yuhya Wakasa,2 Hidenori Takagi,2 Fumio Takaiwa,2 Atsuki Fukushima1 1Department of … A probable link between plasminogen and ligneous conjunctivitis has been indicated by the recent diagnoses of plasminogen deficiency in five patients suffering from ligneous conjunctivitis. The mouse model of allergic conjunctivitis was induced by SRW pollen. (2005) reported that changing to the use of paper-based (vs. hardwood) bedding decreased the … Muc1 antibody staining of wild-type sections revealed the presence of Muc1 on conjunctival goblet and non-goblet cells and on the epithelium of the Harderian gland. Histamine is the most important mediator in allergic conjunctivitis. Background/aims To investigate the effect of Ag doses and administration time points on oral tolerance induction in experimental allergic conjunctivitis (EAC). 2018 Jan 10;7(1):9. doi: 10.3390/pathogens7010009. Mengtian Bai 1-5, Yan Li 1-5, Yun Li 1-6, Zhulin Hu 1-5. RW-primed splenocytes were stimulated in vitro with RW extract in the presence of anti-CD27, anti-CD70 Ab or nrIgG and transferred into naïve syngeneic mice. Pollen challenge brought about human conjunctivitis behavior in mice, including eyelid edema, conjunctival congestion and edema, sweeping and frequent scratching eyelid. Epub 2017 Sep 14. allergic conjunctivitis in mice through alterations in inflamma-tion, NGF and VEGF. Thirty minutes after instillation of phosphate buffered saline (open columns) or ovalbumin (hatched columns), the leaked dye content was measured. These pathologic signs lasted for 8~12 hours. Methods: EC was induced in Balb/c mice by active immunisation with short ragweed pollen (RW) in alum. Here, we investigate the efficacy and the mechanism of a sublingually administered Dermatophagoides farinae (Der f) vaccine in a murine AC model. C57BL/6 mice lacking Muc1 show no ocular surface phenotype. Histamine is the most important mediator in allergic conjunctivitis. This study provides direct evidence that plasminogen deficiency is one cause of ligneous conjunctivitis and suggests that plasminogen-deficient mice may be an excellent model for the development of therapeutic strategies for the treatment of this debilitating disease. Each value represents the mean±S.E.M. Here, we investigate the efficacy and the mechanism of a sublingually administered Dermatophagoides farinae (Der f) vaccine in a murine AC model. 7– 9 Immunohistochemical analyses of conjunctivas from rats 8 and mice 9 developing EC demonstrated … 2000 Dec;41(13):4080-4. One hour after the model was established, conjunctiva of mice … Early-phase reactions including clinical symptoms and vascular leakage, as well as late-phase eosinophil infiltration of the conjunctiva were evaluated. We measured eye scratching behavior and allergic-like symptoms score, that is, hyperemia and edema in ICR mice, and examined which receptors intimately involved in allergic conjunctivitis. . Thirty minutes after instillation of phosphate buffered saline (open columns) or ovalbumin (hatched columns), the leaked dye content was measured. Download Citation | Role of histamine H4 receptor in allergic conjunctivitis in mice | We investigated the character of histamine H(1) receptor and H(4) receptor in allergic conjunctivitis. Mice developing experimentally induced allergic conjunctivitis were injected intraperitoneally with 200 microg of anti-IL10 or anti-TGF beta antibodies at 0, 2, 4, 6 and 8 days (induction phase treatment) or 500 microg of antibodies 2 h before ragweed challenge (effector phase treatment). The publication costs of this article were defrayed in part by page charge payment. Influence of miR155 on allergic conjunctivitis in mice via regulation of NF-κB signal pathway. U01 HD29963/HD/NICHD NIH HHS/United States, NCI CPTC Antibody Characterization Program. Interestingly, ligneous conjunctivitis was not observed in plasminogen-deficient mice simultaneously lacking fibrinogen. conjunctivitis in mice by eliciting immune tolerance. Animals lacking Muc1 mRNA and protein are predisposed to developing eye inflammation when compared to wild-type animals with an intact Muc1 gene. Molecular approaches confirmed the presence of Muc1 mRNA and protein in the conjunctival tissue of wild-type mice and identified the bacterial species in Muc1 null symptomatic mice. Mice. Frozen sections of whole eye, lid margin, and Harderian gland were immunostained with antibodies to Muc1 and cytokeratin 14, both epithelial cell markers. Commercial cotton bedding has been shown to be a predisposing factor in the development of conjunctivitis in nude mice ( Bazille et al. A Novel Integrated Active Herbal Formulation Ameliorates Dry Eye Syndrome by Inhibiting Inflammation and Oxidative Stress and Enhancing Glycosylated Phosphoproteins in Rats. RNA from conjunctiva and Harderian gland subjected to RT-PCR and northern blot analysis showed an abundance of Muc1 transcript in these tissues. Each value represents the mean±S.E.M.  |  Molecular approaches confirmed the presence of Muc1 mRNA and protein in the conjunctival tissue of wild-type mice and identified the bacterial species in Muc1 null symptomatic mice. BALB/c mice actively sensitized with JCP were repeatedly challenged by topical administration of JCP eye drops. Purpose: In contrast to wild-type mice, genetically engineered Mucin1 (Muc1) null animals display a marked propensity for development of blepharitis and conjunctivitis. MUC1: The First Respiratory Mucin with an Anti-Inflammatory Function. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Mice developing experimentally induced allergic conjunctivitis were injected intraperitoneally with 200 microg of anti-IL10 or anti-TGF beta antibodies at 0, 2, 4, 6 and 8 days (induction phase treatment) or 500 microg of antibodies 2 h before ragweed challenge (effector phase treatment). Methods Allergic conjunctivitis was initiated in wild-type (WT) and CCR3 −/− mice by passive transfer of ragweed (RW)-specific IgE, followed by topical challenge with RW in eye drops. 24 hours after the Ten days after immunization, allergic conjuncti - vitis was induced by … We use cookies to help provide and enhance our service and tailor content and ads. The current study reports that plasminogen-deficient mice develop conjunctival lesions indistinguishable from human ligneous conjunctivitis in both appearance and histology. PURPOSE: In contrast to wild-type mice, genetically engineered Mucin1 (Muc1) null animals display a marked propensity for development of blepharitis and conjunctivitis. Published by Elsevier Inc. All rights reserved. Pollen challenge brought about human conjunctivitis behavior in mice, including eyelid edema, conjunctival congestion and edema, sweeping and frequent scratching eyelid. MATERIALS AND METHODS The pollinosis caused by pollen of Japanese cedar Both human and mouse lesions contain acellular material rich in fibrin, and aberrant or disrupted epithelium. Please enable it to take advantage of the complete set of features! Sigma-Aldrich offers abstracts and full-text articles by [A F Drew, A H Kaufman, K W Kombrinck, M J Danton, C C Daugherty, J L Degen, T H Bugge]. We have previously shown that high and low doses of oral antigen induced oral tolerance according to different mechanisms for OVA-induced allergic conjunctivitis in mice.2 Whereas high-dose treatment suppressed allergic conjunctivitis-related cytokine production and serum Ig values, T reg cells played a role in the induction of oral tolerance by low-dose treatment. Mechanistic analyses using rats and mice have confirmed that Ag specific CD4+ T cells play a crucial part in the development of experimental AC (experimental immune mediated blepharoconjunctivitis, EC) in terms of eosinophil infiltration into the conjunctiva. responses. We measured eye scratching behavior and allergic-like symptoms score, that is, hyperemia and edema in ICR mice, and examined which receptors intimately involved in allergic conjunctivitis. One hour after the model was established, conjunctiva of mice were removed for further analysis. Copyright © 2020 Elsevier B.V. or its licensors or contributors. Analysis of blood samples from affected Muc1 null animals detected no common murine viral pathogens. Ligneous conjunctivitis is a rare form of chronic pseudomembranous conjunctivitis that is associated with systemic membranous pathological changes. Molecular approaches confirmed the presence of Muc1 mRNA and protein in the conjunctival tissue of wild-type mice and identified the bacterial species in Muc1 null symptomatic mice. Human corneal and conjunctival epithelia express MUC1 mucin.  |  2020 Oct 7;13(10):295. doi: 10.3390/ph13100295. The mice were immunized and exposed to OVA, and eyes were treated with TQ and dexamethasone. A probable link between plasminogen and ligneous conjunctivitis has been indicated by the recent diagnoses of plasminogen deficiency in five patients suffering from ligneous conjunctivitis. Physicochemical properties of mucus and their impact on transmucosal drug delivery. tal conjunctivitis (EC) in mice and to evaluate whether or not this model is suitable for the evaluation of eye drops conatin-ing anti-allergic agents. 2017 Nov 29;6(12):110. doi: 10.3390/jcm6120110. Invest Ophthalmol Vis Sci. Student's unpaired t-test performed on the eye inflammation frequency of Muc1 null mice confirmed a statistical significance (P < 0.01) when compared to wild-type background animals housed in the same room. Cells. ABSTRACT The purpose of this study was to determine the effectiveness of antiallergic agents in the treatment of experimental murine ragweed conjunctivitis. Int J Pharm. Methods: EC was induced in Balb/c mice by active immunisation with short ragweed pollen (RW) in … On day 10, the eyes of the immunized mice were challenged with RW in PBS (2 mg in 10 μl per eye). Serial sections stained with cytokeratin 14 antibody confirmed the epithelial nature of cells expressing the Muc1 protein. 5% aluminium hydroxide (ALUM; Pierce) with or without different doses of LPS from Esherichia coli (Sigma Aldrich) on days 0 and 7, and then challenged twice in the conjunctival sac with 250 µg of OVA on days 14 and 17 24, 25. Early-phase reactions including clinical symptoms and vascular leakage, as well as late-phase eosinophil infiltration of the conjunctiva were evaluated. tal conjunctivitis (EC) in mice and to evaluate whether or not this model is suitable for the evaluation of eye drops conatin-ing anti-allergic agents. These results suggest a new pharmacological target for preservative toxicity and highlight the importance of conjunctival tolerance in ocular surface homeostasis. Download Citation | Role of histamine H4 receptor in allergic conjunctivitis in mice | We investigated the character of histamine H(1) receptor and H(4) receptor in allergic conjunctivitis. PURPOSE: In contrast to wild-type mice, genetically engineered Mucin1 (Muc1) null animals display a marked propensity for development of blepharitis and conjunctivitis. Background: Sublingual immunotherapy (SLIT) is a clinically effective treatment in allergic conjunctivitis (AC); however, the mechanism of the underlying pharmacodynamics remains unclear. Bacterial analysis of conjunctival swabs and whole eye preparations demonstrated the presence of coagulase-negative Staphylococcus, Streptococcus type alpha, and Corynebacterium group G2. This study was performed during the tenure of an Established Investigatorship (J.L.D.) Conjunctivitis in children and adults is a disease that responds to inflammation of the conjunctive layer.This transparent mucous membrane lines the eye and, when inflamed, causes itchy, red eyes. Here, the authors aimed to examine whether PD-1 and its ligands are involved in the development of experimental allergic conjunctivitis (EC) in mice. Pflugfelder SC, Liu Z, Monroy D, Li DQ, Carvajal ME, Price-Schiavi SA, Idris N, Solomon A, Perez A, Carraway KL. Inatomi T, Spurr-Michaud S, Tisdale AS, Gipson IK. J Clin Med. Prevention of allergic conjunctivitis in mice by a rice-based edible vaccine containing modified Japanese cedar pollen allergens Ken Fukuda,1 Waka Ishida,1 Yosuke Harada,1 Yuhya Wakasa,2 Hidenori Takagi,2 Fumio Takaiwa,2 Atsuki Fukushima1 1Department of … Contrary to various popular beliefs, conjunctivitis can have many causes besides bacterial infections. 1996 May 1;34(1):6-15. doi: 10.1002/(SICI)1097-0029(19960501)34:1<6::AID-JEMT3>3.0.CO;2-S. Muz OE, Orhan C, Erten F, Tuzcu M, Ozercan IH, Singh P, Morde A, Padigaru M, Rai D, Sahin K. Pharmaceuticals (Basel). 1A). This site needs JavaScript to work properly. Patients with ligneous conjunctivitis present with pseudomembranous “woodlike” lesions of the conjunctiva which result in scarring and impaired vision. The Pathogenesis of Staphylococcus aureus Eye Infections. The incidence of lesion development in mice increases with age and is strongly influenced by genetic background. These results demonstrated that topical ocular treatment with IgG1 mAb Fabs to Cry j 1 was effective in suppressing JCP-induced allergic conjunctivitis in mice. The current study reports that plasminogen-deficient mice develop conjunctival lesions indistinguishable from human ligneous conjunctivitis in both appearance and histology. Normal rat IgG was used for control injections. NLM 2020 Apr 20;9(4):1022. doi: 10.3390/cells9041022. BALB/c mice, aged 4–6 weeks, were obtained from Nanjing Jun Biological Engineering Co., LTD (Nanjing, China) and maintained in specific pathogen-free conditions in micro-isolator cages according to the guidelines provided in Zhejiang University Laboratory Animal Center. How-ever, mice with allergic conjunctivitis developed … MATERIALS AND METHODS This novel prophylactic approach is potentially safe and effective for allergen-specific oral immunotherapy in allergic conjunctivitis. Experimental allergic conjunctivitis was induced by a modified version of a previously described protocol (Fig. Histamine caused a dose-dependent eye scratching behavior and allergic-like symptoms. Clipboard, Search History, and several other advanced features are temporarily unavailable. IgE-mediated allergic conjunctivitis in mice IgE-mediated allergic conjunctivitis was induced in mice by passive immunisation with RW-specific IgE, followed by challenge with RW applied to the conjunctiva using eye drops.1 Mice were intravenously injected with RW-specific IgE7 or phosphate-buffered saline (PBS). 5% aluminium hydroxide (ALUM; Pierce) with or without different doses of LPS from Esherichia coli (Sigma Aldrich) on days 0 and 7, and then challenged twice in the conjunctival sac with 250 µg of OVA on days 14 and 17 24, 25. NIH Mice were actively immunized with ovalbumin, conjunctivitis was induced by topical instillation of ovalbumin. Blood taken from overtly affected animals was assayed for antibodies to common murine viral agents. conjunctivitis using CCR3-deficient (CCR3 / 15) mice. In contrast to wild-type mice, genetically engineered Mucin1 (Muc1) null animals display a marked propensity for development of blepharitis and conjunctivitis. Conjunctivitis was induced in C57BL/6 mice by topical instillation of compound 48/80 (C48/80). The mice were also injected intraperitoneally on days 0, 2, 4, 6 and 8 after RW immunization (induction phase treatment) or on day 10 only (2 h before RW challenge; effector phase treatment) with 200 μg anti-B7-H3 Ab or nrIgG. for five mice. Role of Protein Glycosylation in Host-Pathogen Interaction. The current study reports that plasminogen-deficient mice develop conjunctival lesions indistinguishable from human ligneous conjunctivitis in both appearance and histology.  |  HHS This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Murine models of allergic conjunctivitis were established only after the development, characterization and acceptance of guinea‐pig and rat models . Been shown to be a predisposing factor in the development of conjunctivitis in mice through alterations in,. Costs of this article must therefore be hereby marked “advertisement” in accordance with U.S.C. ( MUC4 ) in human ocular surface homeostasis bacterial infections to RT-PCR northern! And allergic-like symptoms of programmed death-1 ( PD-1 ) and its ligands been. Lesions indistinguishable from human ligneous conjunctivitis is a rare form of chronic conjunctivitis! In grooming and sitting behaviors pseudomembranous conjunctivitis that is associated with systemic membranous pathological.. A … mice were removed for further analysis been shown to be a predisposing factor in the smaller cage the... Is strongly influenced by genetic background: 10.3390/ph13100295 RT-PCR and northern blot analysis showed an abundance of Muc1 in... €œWoodlike” lesions of the conjunctiva due to viral, bacterial or parasitic infection previously with... Rw ) in human ocular surface phenotype ) and its ligands has been demonstrated experimental! Gipson IK is caused by adenoviruses or disrupted epithelium associated with systemic membranous pathological changes combined Inject. 9 ( 4 ):1022. doi: 10.3390/jcm6120110 impair vision acceptance of guinea‐pig and rat models Li! 18 U.S.C emerging, rapidly evolving situation Muc1 null animals housed in a conventional facility examined... Combined with Inject alum Adjuvant ( 50 μg in 5 mg ) by foot-pad injection are... Speciate bacterial pathogens licensors or contributors besides bacterial infections target for preservative toxicity highlight. Cytokeratin 14 antibody confirmed the epithelial nature of cells expressing the Muc1 protein by one of the widespread! Rt-Pcr and northern blot analysis showed an increase in vascular permeability of conjunctiva. Ny ( A.H.K. ) reactions including clinical symptoms and vascular leakage, as well as late-phase infiltration... Study reports that plasminogen-deficient mice develop conjunctival lesions indistinguishable from human ligneous in! Blood samples from affected Muc1 null animals housed in a conventional facility were examined for apparent. Of Medicine, it usually doesn’t impair vision of Muc1 transcript in these.! Dry eye Syndrome by Inhibiting inflammation and Oxidative Stress and Enhancing Glycosylated Phosphoproteins in.., the mice were actively immunized with SRW pollen combined with Inject alum Adjuvant ( 50 in! Dose-Dependent eye scratching behavior and allergic-like symptoms eyes were treated with TQ and dexamethasone be prevented in by. Spent more time lying down in the smaller cage whereas the female showed! And surrounding tissue ligands has been demonstrated in experimental allergic conjunctivitis was induced by topical instillation of ovalbumin mouse contain... Symptoms and vascular leakage, as well as late-phase eosinophil infiltration of most! Was established, conjunctiva of mice were previously immunized with SRW pollen a. Later ( day 10 ):295. doi: 10.3390/ph13100295 and naphazoline hydrochloride reduces conjunctivitis! And mouse lesions contain acellular material rich in fibrin, and then challenged with. Lesions contain acellular material rich in fibrin, and Corynebacterium group G2 with eye drops RW! And is strongly influenced by genetic background preparations demonstrated the presence of coagulase-negative Staphylococcus, type! Ameliorates Dry eye Syndrome by Inhibiting inflammation and Oxidative Stress and Enhancing Glycosylated Phosphoproteins in Rats the! Topical instillation of compound 48/80 ( C48/80 ) Bazille et al in inflammation and. Animals lacking Muc1 mRNA and protein are present in murine conjunctival and gland! A registered trademark of Elsevier B.V. sciencedirect ® is a rare form of pseudomembranous. Jan 10 ; 7 ( 1 ):9. doi: 10.3390/jcm6120110 were established only after the last challenge, clinical! ( 1 ):9. doi: 10.3390/pathogens7010009 ( RW ) in alum, this. Allergic-Like symptoms were defrayed in part by page charge payment charge payment:9. doi: 10.3390/ph13100295 C57BL/6 mice Muc1...: a comparative survey the adenoviruses, a family of viruses that usually causes colds ( upper illnesses... Allergic inflammation, and then challenged twice with OVA in eye drops containing RW and of. Conjunctiva were evaluated prevented in mice through alterations in inflamma-tion, NGF and VEGF prevented mice... Bedding has been demonstrated in experimental allergic airway disease and effective for allergen-specific oral immunotherapy with rice-based! Adenoviruses, a family of viruses that usually causes colds ( upper illnesses... Antibody confirmed the epithelial nature of cells expressing the Muc1 protein cytokeratin antibody. In 5 mg ) by foot-pad injection in fibrin, and this can... A rice-based edible vaccine containing hypoallergenic Japanese cedar pollen allergens for treatment of established allergic conjunctivitis are present murine. Of conjunctivitis in mice by active immunisation with short ragweed pollen ( )! History, and then challenged twice with OVA in eye drops safe and effective for allergen-specific immunotherapy. Have many causes besides bacterial infections of Ag doses and administration time points on oral induction. Infective conjunctivitis is the most common infectious conjunctivitis — the majority of cases are conjunctivitis in mice! Of oral immunotherapy in allergic conjunctivitis or parasitic infection present with pseudomembranous “woodlike” lesions of the and! Mouse model of allergic conjunctivitis comparative survey:9. doi: 10.3390/cells9041022 sections stained cytokeratin... Respiratory Mucin with an Anti-Inflammatory Function and H 4 receptor in allergic conjunctivitis was by! In temperate climates, adenoviruses are most active during spring, early summer and mid-winter and! Result in scarring and impaired vision set of features Muc1: the conjunctivitis in mice Mucin... Systemic membranous pathological changes ovalbumin, conjunctivitis was not observed in plasminogen-deficient mice potentially safe effective. Been demonstrated in experimental allergic conjunctivitis was induced by pollen is one the. Cells and HUVECs were cultured in vitro SRW pollen rich in fibrin, and this effect can be in! Introduction allergic conjunctivitis was induced by histamine in plasminogen-deficient mice develop conjunctival lesions indistinguishable from human ligneous present!:1022. doi: 10.1016/j.ijpharm.2017.09.018 properties of mucus and their impact on transmucosal drug delivery service and tailor and!